hct-15 (cl-0097)  (Procell Inc)

Journal: Cell Division

Article Title: Long non-coding RNA NEAT1 induced by BHLHE40 activates Wnt/β-catenin signaling and potentiates colorectal cancer progression

doi: 10.1186/s13008-024-00129-7

Figure Lengend Snippet: BHLHE40 mediates the transcription of NEAT1 in CRC cells. A Genes co-expressed with NEAT1 in CRC were obtained from UALCAN. B The intersection of genes co-expressed with NEAT1 in COAD and READ and the transcription factors targeting NEAT1 downloaded from hTFtarget. C The expression of these eight transcription factors in CRC was analyzed in the UALCAN. D The expression of BHLHE40 in both COAD and READ. E The binding fragment of BHLHE40 on the NEAT1 promoter with the highest score. F RT-qPCR detection of BHLHE40 expression in CRC tissues and adjacent tissues by RT-qPCR (n = 54). G Expression of NEAT1 in CRC cell lines and HCoEpiC cells by RT-qPCR. H Knockdown efficiency of BHLHE40 by RT-qPCR. I RT-qPCR detection of NEAT1 expression after knockdown of BHLHE40 in LoVo and HCT-15 cells. J Changes in luciferase activity after knockdown of BHLHE40 using luciferase activity assay. K BHLHE40 binding to the NEAT1 promoter in CRC cell using ChIP assay. Results were expressed as magnitude of relative expression (means ± SD) from three independent experiments. F Paired t-test; G one-way ANOVA; H – K two-way ANOVA. * p < 0.05

Article Snippet: Human colon epithelium cell HCoEpiC (YS1700C, YaJi Biological, Shanghai, China), and human CRC cell lines LoVo (CL-0144), HCT-15 (CL-0097) and Caco-2 (CL-0050, all from Procell, Wuhan, Hubei, China) were used in this study.

Techniques: Expressing, Binding Assay, Quantitative RT-PCR, Knockdown, Luciferase, Activity Assay

Journal: Cell Division

Article Title: Long non-coding RNA NEAT1 induced by BHLHE40 activates Wnt/β-catenin signaling and potentiates colorectal cancer progression

doi: 10.1186/s13008-024-00129-7

Figure Lengend Snippet: NEAT1 reverses the anti-tumor effects of sh-BHLHE40 in CRC cells. A RT-qPCR detection of NEAT1 overexpression efficiency. B MTT assay for LoVo and HCT-15 cell viability. C Transwell assay for LoVo and HCT-15 cell migration ability. D Transwell assay for LoVo and HCT-15 cell invasion ability. E Western blot for E-cadherin, N-cadherin, and Vimentin expression in LoVo and HCT-15 cells. F Flow cytometry for LoVo and HCT-15 cell apoptosis. Results were expressed as magnitude of relative expression (means ± SD) from three independent experiments. A – F two-way ANOVA. * p < 0.05

Article Snippet: Human colon epithelium cell HCoEpiC (YS1700C, YaJi Biological, Shanghai, China), and human CRC cell lines LoVo (CL-0144), HCT-15 (CL-0097) and Caco-2 (CL-0050, all from Procell, Wuhan, Hubei, China) were used in this study.

Techniques: Quantitative RT-PCR, Over Expression, MTT Assay, Transwell Assay, Migration, Western Blot, Expressing, Flow Cytometry

Journal: Cell Division

Article Title: Long non-coding RNA NEAT1 induced by BHLHE40 activates Wnt/β-catenin signaling and potentiates colorectal cancer progression

doi: 10.1186/s13008-024-00129-7

Figure Lengend Snippet: NEAT1 reverses the metastasis-suppressing and tumor-suppressing properties of sh-BHLHE40. HCT-15 cells after infection were injected subcutaneously into nude mice to observe tumor growth. A Tumor growth curve. B Immunohistochemical analysis of BHLHE40 and Ki67 expression in xenograft tumor tissues of nude mice. C HCT-15 cells after infection were injected into nude mice via the tail vein, and lung metastasis formation was observed using HE staining. D Western blot for BHLHE40 and EMT-related protein expression in metastatic tissues with lung infiltration. Results were expressed as magnitude of relative expression (means ± SD) from six nude mice in each group. Two-way ANOVA. * p < 0.05

Article Snippet: Human colon epithelium cell HCoEpiC (YS1700C, YaJi Biological, Shanghai, China), and human CRC cell lines LoVo (CL-0144), HCT-15 (CL-0097) and Caco-2 (CL-0050, all from Procell, Wuhan, Hubei, China) were used in this study.

Techniques: Infection, Injection, Immunohistochemical staining, Expressing, Staining, Western Blot

Journal: Cell Division

Article Title: Long non-coding RNA NEAT1 induced by BHLHE40 activates Wnt/β-catenin signaling and potentiates colorectal cancer progression

doi: 10.1186/s13008-024-00129-7

Figure Lengend Snippet: BHLHE40 mediates NEAT1 transcription to activate Wnt/β-catenin signaling in CRC cells. A Western blot for the E-cadherin, N-cadherin, Vimentin, Wnt, β-catenin, c-myc, and cyclin D1 protein expression in CRC cells in response to oe-BHLHE40 (oe-NC as control) or oe-BHLHE40 + sh-NEAT1 (oe-BHLHE40 + sh-NC as control). B Effects of oe-BHLHE40 and iCRT3 combined treatment on Wnt/β-catenin pathway activity in CRC cells was measured using TOP/FOP flash assay. C MTT assay for LoVo and HCT-15 cell viability. D Transwell assay for LoVo and HCT-15 cell migration ability. E Transwell assay for LoVo and HCT-15 cell invasion ability. F Flow cytometry for LoVo and HCT-15 cell apoptosis. Results were expressed as magnitude of relative expression (means ± SD) from three independent experiments. Two-way ANOVA. * p < 0.05

Article Snippet: Human colon epithelium cell HCoEpiC (YS1700C, YaJi Biological, Shanghai, China), and human CRC cell lines LoVo (CL-0144), HCT-15 (CL-0097) and Caco-2 (CL-0050, all from Procell, Wuhan, Hubei, China) were used in this study.

Techniques: Western Blot, Expressing, Control, Activity Assay, MTT Assay, Transwell Assay, Migration, Flow Cytometry

Journal: Cell Division

Article Title: Long non-coding RNA NEAT1 induced by BHLHE40 activates Wnt/β-catenin signaling and potentiates colorectal cancer progression

doi: 10.1186/s13008-024-00129-7

Figure Lengend Snippet: BHLHE40 mediates the transcription of NEAT1 in CRC cells. A Genes co-expressed with NEAT1 in CRC were obtained from UALCAN. B The intersection of genes co-expressed with NEAT1 in COAD and READ and the transcription factors targeting NEAT1 downloaded from hTFtarget. C The expression of these eight transcription factors in CRC was analyzed in the UALCAN. D The expression of BHLHE40 in both COAD and READ. E The binding fragment of BHLHE40 on the NEAT1 promoter with the highest score. F RT-qPCR detection of BHLHE40 expression in CRC tissues and adjacent tissues by RT-qPCR (n = 54). G Expression of NEAT1 in CRC cell lines and HCoEpiC cells by RT-qPCR. H Knockdown efficiency of BHLHE40 by RT-qPCR. I RT-qPCR detection of NEAT1 expression after knockdown of BHLHE40 in LoVo and HCT-15 cells. J Changes in luciferase activity after knockdown of BHLHE40 using luciferase activity assay. K BHLHE40 binding to the NEAT1 promoter in CRC cell using ChIP assay. Results were expressed as magnitude of relative expression (means ± SD) from three independent experiments. F Paired t-test; G one-way ANOVA; H – K two-way ANOVA. * p < 0.05

Article Snippet: Human colon epithelium cell HCoEpiC (YS1700C, YaJi Biological, Shanghai, China), and human CRC cell lines LoVo (CL-0144), HCT-15 (CL-0097) and Caco-2 (CL-0050, all from Procell, Wuhan, Hubei, China) were used in this study.

Techniques: Expressing, Binding Assay, Quantitative RT-PCR, Knockdown, Luciferase, Activity Assay

Journal: Cell Division

Article Title: Long non-coding RNA NEAT1 induced by BHLHE40 activates Wnt/β-catenin signaling and potentiates colorectal cancer progression

doi: 10.1186/s13008-024-00129-7

Figure Lengend Snippet: NEAT1 reverses the anti-tumor effects of sh-BHLHE40 in CRC cells. A RT-qPCR detection of NEAT1 overexpression efficiency. B MTT assay for LoVo and HCT-15 cell viability. C Transwell assay for LoVo and HCT-15 cell migration ability. D Transwell assay for LoVo and HCT-15 cell invasion ability. E Western blot for E-cadherin, N-cadherin, and Vimentin expression in LoVo and HCT-15 cells. F Flow cytometry for LoVo and HCT-15 cell apoptosis. Results were expressed as magnitude of relative expression (means ± SD) from three independent experiments. A – F two-way ANOVA. * p < 0.05

Article Snippet: Human colon epithelium cell HCoEpiC (YS1700C, YaJi Biological, Shanghai, China), and human CRC cell lines LoVo (CL-0144), HCT-15 (CL-0097) and Caco-2 (CL-0050, all from Procell, Wuhan, Hubei, China) were used in this study.

Techniques: Quantitative RT-PCR, Over Expression, MTT Assay, Transwell Assay, Migration, Western Blot, Expressing, Flow Cytometry

Journal: Cell Division

Article Title: Long non-coding RNA NEAT1 induced by BHLHE40 activates Wnt/β-catenin signaling and potentiates colorectal cancer progression

doi: 10.1186/s13008-024-00129-7

Figure Lengend Snippet: BHLHE40 mediates NEAT1 transcription to activate Wnt/β-catenin signaling in CRC cells. A Western blot for the E-cadherin, N-cadherin, Vimentin, Wnt, β-catenin, c-myc, and cyclin D1 protein expression in CRC cells in response to oe-BHLHE40 (oe-NC as control) or oe-BHLHE40 + sh-NEAT1 (oe-BHLHE40 + sh-NC as control). B Effects of oe-BHLHE40 and iCRT3 combined treatment on Wnt/β-catenin pathway activity in CRC cells was measured using TOP/FOP flash assay. C MTT assay for LoVo and HCT-15 cell viability. D Transwell assay for LoVo and HCT-15 cell migration ability. E Transwell assay for LoVo and HCT-15 cell invasion ability. F Flow cytometry for LoVo and HCT-15 cell apoptosis. Results were expressed as magnitude of relative expression (means ± SD) from three independent experiments. Two-way ANOVA. * p < 0.05

Article Snippet: Human colon epithelium cell HCoEpiC (YS1700C, YaJi Biological, Shanghai, China), and human CRC cell lines LoVo (CL-0144), HCT-15 (CL-0097) and Caco-2 (CL-0050, all from Procell, Wuhan, Hubei, China) were used in this study.

Techniques: Western Blot, Expressing, Control, Activity Assay, MTT Assay, Transwell Assay, Migration, Flow Cytometry